The Reaction of Cellulolytic and Potentially Cellulolytic Spore-Forming Bacteria to Various Types of Crop Management and Farmyard Manure Fertilization in Bulk Soil

The ecology of cellulolytic bacteria in bulk soil is still relatively unknown. There only a handful papers on the abundance and diversity this group bacteria. Our study aimed to determine impact various crop management systems farmyard manure (FYM) fertilization potentially spore-forming (SCB). site was nearly 100-year-old experiment, one oldest active field trials Europe. highest contents total carbon (TC) nitrogen (TN) were recorded both five-year rotations. abundances SCB potential evaluated using classical microbiological methods, most probable number (MPN), 16S rRNA Illumina MiSeq sequencing. MPN with arbitrary rotation without legumes (ARP) fertilized FYM (382 colony-forming units (CFU) mL−1). As result bioinformatic analysis, values Shannon–Wiener index largest operational taxonomic (OTUs) found ARP-FYM, while lowest ARP treatment fertilization. In all treatments, those dominant at order level were: Brevibacillales (13.1–43.4%), Paenibacillales (5.3–36.9%), Bacillales (4.0–0.9%). Brevibacillaceae Paenibacillaceae (8.2–36.9%), Clostridiaceae (5.4–11.9%) dominated family tested samples. Aneurinibacillaceae Hungateiclostridiaceae families increased their overall share treatments. results our research show that types negligible actual factor shaping community use